Examinando por Autor "Bernilla Carrillo, Diana"

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Motility performance of thawed spermatozoa of bulls from the tropics throughout the year
(Animals, 2025-08-21) Poclín Rojas, Annie Yoselin; Arbaiza Barnechea, Martín Daniel; Segura Portocarrero, Gleni Tatiana; Ampuero Trigoso, Gustavo; Bernilla Carrillo, Diana; Depaz Hizo, Benjamín Alberto; Vásquez Tarrillo, Ronald Will; Diaz Quevedo, Clavel; Quispe Ccasa, Hurley Abel
Under tropical conditions, seasonal variations may also influence the sperm characteristics of Bos indicus. The objective was to evaluate the motility of thawed sperm of bulls from the Peruvian tropics throughout the year. Over 24 months, 129 ejaculates were evaluated based on semen quality and subjected to slow horizontal freezing in 0.5 mL straws. After thawing, the individual, seasonal period, and season effect on motility and kinetic parameters were analyzed using a Sperm Class Analyzer® (Microptic S.L.U., Barcelona, Spain). There was an individual effect on volume, motility, fresh concentration, and kinetic parameters when thawed. In the dry period, higher straight-line velocity (VSL) (p < 0.05) and beat cross frequency (BCF) were found than in the rainy period (p < 0.01). In summer and autumn, there was greater total motility, fast, circular routes, curvilinear velocity, average path velocity, VSL, amplitude of lateral displacement of the head, and BCF (p < 0.01). Greater volume and motility were found in winter and spring, but in summer and autumn, greater speed and vigor of movement were obtained in thawed sperm. The variation in annual climate patterns influences the seminal quality of bulls, and its effect needs to be assessed to propose adaptation strategies to climate change in tropical areas.
Use intensity of progesterone devices for fixed-time artificial insemination in gyr crossbred cows from the peruvian tropics
(Agricultural Research Communication Centre (ARCC), 2025-01-13) Poclín Rojas, Annie Yoselin; Segura, Gleni Tatiana; Bernilla Carrillo, Diana; Ampuero Trigoso, Gustavo; Quispe Ccasa, Hurley Abel; Vasquez Tarrillo, Ronald Will; Depaz Hino, Benjamin; Saucedo Uriarte, José Américo
Background: The implementation of routine fixed-time artificial insemination programs can generate significant waste and costs, which good management practices can help mitigate. The objective of the study was to assess the effects of second- and third-use progesterone devices on estrus characteristics and pregnancy rates in synchronized crossbred Gyr cows. Methods: Ten cows were synchronized with first-use Bovine Intravaginal Progesterone Devices (DIB), nine with second-use DIB and ten with third-use DIB. All cows were ≥90 days postpartum, had an average milk production of 6.9±1.9 L/day and were aged 4-10 years. Estrus signs were recorded at insemination and pregnancies were confirmed at 60 days by ultrasonography. Result: No significant associations were found between DIB use intensity and estrus detection patch response, mucus color, body condition score (CC), calvings, or breed purity (p>0.05). Body condition was associated with uterine turgor tone (p<0.05) and 100% of cows with CC 3.0 showed turgor tone 3. The highest pregnancy rate was found in second-use DIB (55.6%) and CC 2.8 (47.1%), though neither association was significant (p>0.05). DIBs may be used up to a second-use, as cervical mucosal stimulation and pregnancy rate remain unaffected.