Capítulos de Libros

URI permanente para esta colecciónhttps://repositorio.inia.gob.pe/handle/20.500.12955/1935

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Bioprospecting and exploration of extremophilic enzymes in bioremediation of wastewater polluted
(CRC Press, 2024-04-12) Hualpa Cutipa, Edwin; Solorzano Acosta, Andi; Alfaro Cancino, Milagros Estefani; Arquíñego Zárate, Fiorella Maité; Julca Santur, Nikol Gianella; Mayhua, María José; Castro Tena, Lucero Katherine
Water, covering three-quarters of the Earth, is crucial for life and ecosystems and is also essential in industrial processes. However, most aquatic environments are polluted due to the massive discharge of wastewater with high pollutant loads, which threatens ecosystem health. To mitigate this environmental impact, humans have tried various physical and chemical strategies, but increased wastewater and reagents have the disadvantage of generating more waste, making it an environmentally unsustainable problem. Governments are now focusing on integrating living organisms or biological treatments to reduce or mitigate pollutants in contaminated matrices. Microbial biodiversity is being studied through bioprospecting strategies and the search for new microorganisms capable of biodegrading pollutants. Extremophilic microbes, which have undergone evolutionary processes and exhibited adaptive traits, have the potential to transform harmful contaminants into beneficial resources and improve their tolerance mechanisms to complex pollutants. This chapter aims to provide updated information on sustainable wastewater bioremediation processes using extremophilic microorganisms and their enzymes, considering their properties, characteristics, stability, and biodegradation capacity. Genetic modifications and editions are also discussed to obtain biomolecules with greater versatility and efficacy
Omics strategies targeting microbes with microplastic detection and biodegradation properties
(CRC Press, 2024-04-12) Hualpa Cutipa, Edwin; Solórzano Acosta, Andi; Ravelo Machari, Yadira Karolay; Gomez Barrientos, Fiorella; Huayllacayan Mallqui, Jorge Johnny; Arquíñego Zárate, Fiorella Maité; León Chacón, Andrea; Alfaro Cancino, Milagros Estefani
Plastic-based products are ubiquitous in ecosystems due to their widespread use and utility in everyday life. Water matrices and winds are the primary means of plastic dispersal, which poses a threat to water consumption due to the presence of invisible contaminants. Microplastics (MPs) are the result of the physical, chemical, and biological degradation of bulky plastics, becoming a health hazard to living organisms. Microorganisms play a crucial role in the recycling and decomposition of harmful compounds. Studies have explored the properties of microorganisms in biodegradation and genetic manipulation to improve their metabolic properties. However, new strategies for detecting and understanding MPs’ metabolic versatility are needed. Various techniques have been developed to detect microbes and their biomolecules in various environments, including those not detectable using traditional culturing techniques. Omics approaches based on massive sequencing technologies (next generation sequencing) provide a better understanding of microbial cells and their characteristics. Strategies such as genomics, transcriptomics, metagenomics, proteomics, and metabolomics allow for precise molecular analysis, generating information about the genes a microorganism can harbor and the pattern of gene expression and protein synthesis under different conditions. This chapter aims to address the main omics strategies for detecting and identifying MPs and their biomolecules, including recent studies in humans.

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Examinando Capítulos de Libros por Autor "Arquíñego Zárate, Fiorella Maité"